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The Institute for International Cooperation in Animal Biologics (IICAB) hosted five “USDA Highly Pathogenic Avian Influenza Diagnostic Training Courses” in 2006-2007 that were funded by the USDA. The purpose was to teach diagnosticians from laboratories throughout the world how to diagnose avian influenza and to build a network of colleagues in this field. In 2005, the IICAB hosted a similar course, funded by the USDA Agriculture Research Service and focused on diagnosing avian influenza and exotic Newcastle disease. The courses were taught by scientists from the USDA APHIS National Veterinary Services Laboratories and the USDA ARS Southeast Poultry Research Laboratory. View attendee list and photographs here!

The five day course has been recorded and includes lectures on avian influenza and Newcastle disease viruses, types of diagnostic tests, molecular diagnostics, agar gel immunodiffusion, virus characterization, and sample collection, preservation and shipping. Detailed information on laboratory tests are shown along with printable resources to help viewers follow each step in the procedures. Additional printable resources on avian influenza are included as well. For more information contact the Institute for International Cooperation in Animal Biologics at iicab@iastate.edu.

	Overview of Avian Influenza Dr. David Suarez Duration: 49 min. [View Video] Slide Presentation: [PPT] [PDF]

	Overview of Newcastle Disease Virus Mr. Dennis Senne Duration: 58 min. [View Video] Slide Presentation: [PPT] [PDF]

	Laboratory Orientation and Safety Mr. Dennis Senne Duration: 26 min. [View Video] Slide Presentation: [PPT] [PDF] Biosafety in Microbiological and Biomedical Laboratories (BMBL) 4th Edition: [HTM]

	Laboratory Diagnosis of Avian Influenza and Newcastle Disease Mr. Dennis Senne Duration: 54 min. [View Video] Slide Presentation: [PPT] [PDF]

	Application of Real Time RT-PCR to Detect Avian Influenza Virus Dr. David Suarez Duration: 56 min. [View Video] Slide Presentation: [PPT] [PDF]

	Characterization and Pathotyping of AIV and APMV-1 Mrs. Jan Pedersen Duration: 35 min. [View Video] Slide Presentation: [PPT] [PDF]

	Recommended Procedures for Sample Collection, Preservation and Shipping Mr. Dennis Senne Duration: 34 min. [View Video] Slide Presentation: [PPT] [PDF]

Avian Influenza Lab Demonstrations: All The lab demonstrations are accompanied by the following reference guide which can be used to follow along with the video: Lab Demonstration Reference Guide (PDF 16.4MB)
	All Lab Demonstrations (listed below) USDA Duration: 37 min. [View Video]

Avian Influenza Lab Demonstrations: Virus Isolation The demonstrations listed below are accompanied by the following reference material: Virus Isolation and Specimen Processing: [PPT] [PDF]
	Biological Safety Cabinets USDA Duration: 1 min. 19 sec. [View Video]

	Tracheal Swabs USDA Duration: 45 sec. [View Video]

	Oropharyngeal Swabs USDA Duration: 30 sec. [View Video]

	Cloacal Swabs USDA Duration: 37 sec. [View Video]

	Tissue Samples USDA Duration: 2 min. 7 sec. [View Video]

	Candling Eggs USDA Duration: 1 min. 11 sec. [View Video]

	Egg Inoculation USDA Duration: 1 min. 3 sec. [View Video]

	AAF Collection (Dead Embryos) USDA Duration: 1 min. 28 sec. [View Video]

	AAF Collection (Live Embryos) USDA Duration: 1 min. 30 sec. [View Video]

Avian Influenza Lab Demonstrations: HA and HI The demonstrations listed below are accompanied by the following reference material: Detection and Identification of Hemagglutinating Isolates: [PPT] [PDF] The following protocols are available. Please contact Dennis Senne or Jan Pedersen by email or phone (515-663-7266). USDA NVSL Testing Protocol HI Test for Subtype Identification of Influenza A Virus Antibody HI Test to Detect Serum Antibodies to Avian Paramyxoviruses HA and HI Tests for Avian Paramyxovirus (APMV) Identification USDA NVSL Standard Operating Procedure Preparation of Erythrocytes for HA and HI Tests Removal of Natural Agglutinins from Avian Serums
	HA Test USDA Duration: 3 min. 57 sec. [View Video]

	HA Test Results USDA Duration: 57 sec. [View Video]

	HA Back Titration USDA Duration: 2 min. 5 sec. [View Video]

	HI Test USDA Duration: 3 min. 37 sec. [View Video]

Avian Influenza Lab Demonstrations: ELISA The demonstration listed below is accompanied by the following reference material: ELISA: [PPT] [PDF] Laboratory Handout: [PDF]
	IDEXX ELISA USDA Duration: 3 min. 18 sec. [View Video]

Avian Influenza Lab Demonstrations: Antigen Detection The demonstation listed below is accompanied by the following reference material: AIV Antigen Detection: [PPT] [PDF] Laboratory Handout: [PDF]
	Antigen Detection USDA Duration: 1 min. 31 sec. [View Video]

Avian Influenza Lab Demonstrations: rRT-PCR The demonstations listed below are accompanied by the following reference material: Recommended Procedures for the Extration of RNA: [PPT] [PDF] Amplification and Detection of Nucleic Acid by the Real-Time RT-PCR Procedure: [PPT] [PDF] Interpretation of Results: [PPT] [PDF] rRT-PCR Lab Handouts: [PDF] NVSL Testing Protocol The following two protocols are available. Please contact Dennis Senne or Jan Pedersen by email or phone (515-663-7266). Real-Time RT-PCR for Detection of Virulent Newcastle Disease Virus in Clinical Samples Real-Time RT-PCR for Detection of Avian Influenza Virus and Identification of H5 and H7 subtypes in Clinical Samples
	Ambion^® Magnetic Bead Extraction USDA Duration: 3 min. 47 sec. [View Video]

	Qiagen^® RNeasy^® Mini Columns USDA Duration: 4 min. 6 sec. [View Video]

	Master Mix Preparation USDA Duration: 3 min. 22 sec. [View Video]
Other Resources Distributed to Course Participants Highly Pathogenic Avian Influenza OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals 2004 (Chapter 2.1.14): [PDF] OIE Terrestrial Animal Health Code 2004 (Chapter 2.7.12): [PDF] Newcastle Disease OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals 2004 (Chapter 2.1.15): [PDF] OIE Terrestrial Animal Health Code 2004 (Chapter 2.7.13): [PDF]